Main

Fields of interest:

• Biosynthesis of haem and chlorophyll
• Bacterial redox homeostasis
• Bacterial globins and nitrosative stress in Campylobacter jejuni

Recent publications:

M. Shepherd, V. Barynin, C. Lu, P.V. Bernhardt , G. Wu, S.R. Yeh, T. Egawa, S.E. Sedelnikova, D.W. Rice, J.L. Wilson, and R.K. Poole:
The single-domain globin from the pathogenic bacterium Campylobacter jejuni: Novel D-Helix conformation, proximal hydrogen bonding that influences ligand binding, and peroxidase-like redox properties,
J. Biol. Chem., (2010), In press

M. Shepherd and H.A. Dailey:
Peroxidase activity of cytochrome c facilitates the protoporphyrinogen oxidase reaction,
Cell. Mol. Biol., Vol. 55, (2009), pp. 6-14

M.G. Mason, M. Shepherd, P. Nicholls, P.S. Dobbin, K.S. Dodsworth, R.K. Poole and C.E. Cooper:
Cytochrome bd confers nitric oxide resistance to Escherichia coli,
Nature Chemical Biology, Vol 5, (2009), pp. 94-96

M.G. Mason, R.S. Holladay, P. Nicholls, M. Shepherd and C.E. Cooper:
Quantitative analysis of nitric oxide inhibition of cytochrome oxidases of the respiratory chain,
Meth. Enzymol., Vol 437, (2008), 135-159

M. Shepherd, M.D. Heath and R.K. Poole:
NikA binds heme: A new role for an Escherichia coli periplasmic nickel-binding protein,
Biochemistry, Vol. 46, (2007), pp. 5030-5037

M. Shepherd, T.A. Dailey and H.A. Dailey:
A New Class of `2Fe-2S` Cluster-Containing Protoporphyrin IX Ferrochelatases,
Biochem. J., Vol. 397, (2006), pp. 47-52

M. Shepherd, S. Mclean and C.N. Hunter:
Kinetic basis for linking the first two enzymes of chlorophyll biosynthesis,
FEBS J., Vol. 272, (2005), pp. 4532-4539

M. Shepherd and H.A. Dailey:
A continuous fluorimetric assay for protoporphyrinogen oxidase by monitoring porphyrin accumulation,
Anal. Biochem., Vol. 344, (2005), pp. 115-121

M. Shepherd and C.N. Hunter:
Transient kinetics of the reaction catalysed by magnesium protoporphyrin IX methyltransferase,
Biochem. J., Vol. 382, (2004), pp. 1009-1013

M. Shepherd, J.D. Reid and C.N. Hunter:
Purification and kinetic characterization of the magnesium protoporphyrin IX methyltransferase from Synechocystis PCC6803,
Biochem. J., Vol. 371, (2003), pp. 351-360