Main

Fields of interest:

• DNA methylation
• Methyltransferase mechanisms
• Enzyme/cofactor engineering
• Targeted labeling of biopolymers

Recent publications:

S. Klimašauskas and G. Lukinavičius:
Chemistry of AdoMet-dependent methyltransferases,
Wiley Encyclopedia of Chemical Biology, (2008), in press,
doi: 10.1002/9780470048672.wecb335

G. Lukinavičius, V. Lapienė, Z. Staševskij, C. Dalhoff, E. Weinhold and S. Klimašauskas:
Targeted labeling of DNA by methyltransferase-directed Transfer of Activated Groups (mTAG),
J. Amer. Chem. Soc., Vol. 129, (2007), pp. 2758-2759.

S. Klimašauskas and E. Weinhold:
A new tool for biotechnology: AdoMet-dependent methyltransferases,
Trends Biotechnol., Vol. 25, (2007), pp. 99-104.

Z. Yang, G. Vilkaitis, B. Yu, S. Klimašauskas and X. Chen:
Approaches for studying microRNA and small interfering RNA methylation in vitro and in vivo,
Methods Enzymol., Vol. 247, (2007) pp. 139-154.

A. Sevostyanova, A. Feklistov, N. Barinova, E. Heyduk, I. Bass, S. Klimašauskas, T. Heyduk and A. Kulbachinskiy:
Specific recognition of the -10 promoter element by the free RNA polymerase sigma subunit,
J. Biol. Chem., Vol. 282, (2007), pp. 22033-22039.

O.M. Subach, D.V. Maltseva, A. Shastry, A. Kolbanovskiy, S. Klimašauskas, N.E. Geacintov, and E.S. Gromova:
The stereochemistry of benzo[]pyrene-2'-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases,
FEBS J., Vol. 274, (2007), pp. 2121-2134.

C. Dalhoff, G. Lukinavičius, S. Klimašauskas and E. Weinhold:
Synthesis of S-adenosyl-L-methionine analogs and their use for sequence-specific transalkylation of DNA by methyltransferases,
Nat. Protocols, Vol. 1, (2006), pp. 1879-1886.

A. Feklistov, N. Barinova, A. Sevostyanova, E. Heyduk, I. Bass, I. Vvedenskaya, K. Kuznedelov, E. Merkiene, E. Stavrovskaya, S. Klimašauskas, V. Nikiforov, T. Heyduk, K. Severinov and A. Kulbachinskiy:
A basal promoter element recognized by free RNA polymerase sigma subunit determines promoter recognition by RNA polymerase holoenzyme,
Mol. Cell, Vol. 23, (2006), pp. 97-107.

C. Dalhoff, G. Lukinavičius, S. Klimašauskas and E. Weinhold:
Direct transfer of extended groups from synthetic cofactors by DNA methyltransferases,
Nat. Chem. Biol., Vol. 2, (2006), pp. 31-32.

E. Merkiene and S. Klimašauskas:
Probing a rate-limiting step by mutational perturbation of AdoMet binding in the HhaI methyltransferase,
Nucleic Acids Res., Vol. 33, (2005), pp. 307-315.