Main

Fields of interest:

• Alpha-amylase family enzymes as a clan GH-H of glycoside hydrolases - sequences, structures, specificities, stability and evolution
• Starch-binding domains as carbohydrate-binding module families - similarities and relationships among them.
• The family GH31 of glycoside hydrolases and remote sequence homologies shared with the alpha-amylase family.
• The family GH57 of glycoside hydrolases as an extremely diverged protein family.
• TIM-barrel proteins and enzymes - as examples of the most frequently occurring protein folding motif.
• Protein evolution versus taxonomic relationships among different organisms from Bacteria, Archaea and Eucarya.

Recent publications:

S. Janecek, B. Svensson and E.A. MacGregor:
A remote but significant sequence homology between glycoside hydrolase clan GH-H and family GH31.
FEBS Lett., Vol. 581, (2007), pp. 1261-1268.

M. Machovic and S. Janecek:
Starch-binding domains in the post-genome era.
Cell. Mol. Life Sci., Vol. 63,  (2006), pp. 2710-2724.

M. Machovic, B. Svensson, E.A. MacGregor and S. Janecek:
A new clan of CBM families based on bioinformatics of starch-binding domains from families CBM20 and CBM21.
FEBS J., Vol. 272, (2005), pp. 5497-5513.

R. Zona, F. Chang-Pi-Hin, M.J. O'Donohue and S. Janecek:
Bioinformatics of the family 57 glycoside hydrolases and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis.
Eur. J. Biochem., Vol. 271, (2004), pp. 2863-2872.

S. Janecek, B. Svensson and E.A. MacGregor:
Relation between domain evolution, specificity, and taxonomy of the α-amylase family members containing a C-terminal starch-binding domain.
Eur. J. Biochem., Vol. 270, (2003), pp. 635-645.

A. Oslancova and S.Janecek:
Oligo-1,6-glucosidase and neopullulanase enzyme subfamilies from the α-amylase family defined by the fifth conserved sequence region.
Cell. Mol. Life Sci., Vol. 59, (2002), pp. 1945-1959.

S. Janecek:
A motif of a microbial starch-binding domain found in human genethonin.
Bioinformatics, Vol. 18, (2002), pp. 1534-1537.

E.A. MacGregor, S. Janecek and B. Svensson:
Relationship of sequence and structure to specificity in the α-amylase family of enzymes.
Biochim. Biophys. Acta, Vol. 1546, (2001), pp. 1-20.

S. Janecek, B. Svensson, and R.R.B. Russell:
Location of repeat elements in glucansucrases of Leuconostoc and Streptococcus species.
FEMS Microbiol. Lett., Vol. 192, (2000), pp. 53-57.

S. Janecek and J. Sevcik:
The evolution of starch-binding domain.
FEBS Lett., Vol. 456, (1999), pp. 119-125.

S. Janecek, E. Leveque, A. Belarbi and B. Haye:
Close evolutionary relatedness of α-amylases from Archaea and plants.
J. Mol. Evol., Vol. 48, (1999), pp. 421-426.

S. Janecek, B. Svensson and B. Henrissat:
Domain evolution in the α-amylase family.
J. Mol. Evol., Vol. 45, (1997), pp. 322-331.